Effect of growth regulators on shoot proliferation and root development in micropropagation of pomegranate (Punica granatum L.)

In this study the effect of growth regulators in different concentrations were tested for shoot and root development micropropagation pomegranate with nodal explants. The explants collected morning, third node from tip. healthy plants which authenticated. For MS media was supplemented alpha-naphthalene acetic acid (NAA), indole-3-butyric-acid (IBA), 6-benzyl aminopurine (BAP) kinetin their combinations. rot (IBA) tested. It is observed that when ADS 15 mg/l + NAA 2.0 BAP had given highest maximum proliferation, number shoots, leaves along nodes intermodal length survival per cent, followed by 1.0 mg/l. IBA 0.6 roots shoot, shoots producing more new NAA.